Brain vitrification preservation program
preclinicalresearch program · high · Wed Jan 28 2026 00:00:00 GMT+0000 (Coordinated Universal Time)
Preserve mammalian and human brain ultrastructure by vitrification without prior aldehyde fixation, supporting brain preservation and biostasis research.
Perfusion with the M22 vitrification solution followed by vitrification, warming, fixation, biopsy evaluation, dilution, histology, and electron microscopy.
2026 bioRxiv report showed rabbit and human brain tissue could be vitrified with predominant ultrastructural integrity and no visible ice damage under tested conditions.
Rabbit brains perfused with M22 showed absence of visible ice damage and overall structural preservation, though with osmotic shrinkage; human cortical biopsies after whole-brain M22 perfusion showed predominantly intact cells, neuropil, and synapses without ice crystal damage.
Brain Preservation Prize preservation demonstrations
preclinicalresearch program · medium · Tue Mar 13 2018 00:00:00 GMT+0000 (Coordinated Universal Time)
Demonstrate high-quality mammalian brain preservation sufficient to win independent brain preservation prizes.
Brain preservation methods using cryopreservation/vitrification techniques; supplied record does not detail the exact protocol.
Won the Small Mammal Brain Preservation Prize on 2016-02-09 and the Large Mammal Brain Preservation Prize on 2018-03-13.
Public record states 21st Century Medicine won both small and large mammal brain preservation prizes.
Heart preservation research grant program
preclinicalresearch program · medium · Sun Oct 31 2004 00:00:00 GMT+0000 (Coordinated Universal Time)
Study a preservation solution for extending simple cold storage time of human hearts removed for transplant.
NIH-funded research evaluating a University of Rochester preservation solution for donor heart cold storage.
Received a $900,000 NIH grant in 2004 to study heart preservation for transplantation.
Supplied material reports the grant and objective, but not experimental outcomes.
Human biostasis research roadmap
exploratoryresearch program · medium
Define research directions to improve human biostasis, including preservation protocols, quality metrics, stabilization strategies, and long-term preservation methods.
Roadmap paper synthesizing challenges and proposed research directions for human biostasis and potential future revival technologies.
2024 Brain Sciences publication describes a roadmap intended for biennial updates.
The roadmap emphasizes that reversible human preservation is not yet possible and prioritizes high-fidelity brain preservation and metrics for preservation quality.
Hypothermic preservation and cryopreservation platform
undisclosedplatform · high
Enable protection, preservation, transportation, storage, and future use of valuable living systems across biopharmaceuticals, assisted reproductive technology, transplantation, and related bio-artificial applications.
Platform technology using hypothermic preservation and cryopreservation techniques for living systems; includes lab-scale cryopreservation solutions for research and educational purposes.
Company site describes the platform as developed and applied across multiple preservation markets.
Public materials describe a broad preservation platform but do not provide quantified commercial or clinical results.
Kidney vitrification and nanowarming research
preclinicalresearch program · medium
Improve recovery of vitrified kidneys by rapidly and uniformly rewarming organs to avoid ice crystallization and cracking.
Perfusion of rat kidneys with cryoprotective cocktail and silica-coated iron oxide nanoparticles, vitrification, microcomputed tomography verification, and radiofrequency nanowarming.
2021 Advanced Science study reported successful recovery of a rat kidney from the vitrified state using nanowarming.
Nanowarmed kidneys showed avoidance of ice crystallization and cracking in experiments and modeling, and histology/confocal imaging were dramatically better than convective rewarming controls.
Partial freezing liver preservation research
preclinicalresearch program · medium
Extend liver preservation time by inducing a stable high-subzero partial freezing state while maintaining enough unfrozen fraction to limit ice-mediated injury.
Rat liver partial freezing at -10 °C to -15 °C using glycerol as the main permeating cryoprotectant, with testing of ice modulators including antifreeze glycoprotein and polyvinyl alcohol/polyglycerol X/Z-1000.
2022 Frontiers in Physics study compared glycerol-only, AFGP, and X/Z-1000 formulations in partially frozen rat livers.
AFGP livers had high ATP and least edema but endothelial damage; X/Z-1000 livers had highest ATP and energy charge but endothelial damage and edema; glycerol-only controls had least DNA damage but lowest ATP and energy charge.
Rabbit kidney vitrification program
preclinicalresearch program · medium
Demonstrate vitrification of a kidney at cryogenic temperatures as a step toward organ banking and transplant preservation.
Kidney vitrification to -135 °C using 21st Century Medicine cryopreservation methods; supplied record does not provide full protocol details.
Announced vitrification of a rabbit kidney to -135 °C at the July 2005 Society for Cryobiology annual conference.
Supplied public record reports the vitrification milestone but does not include functional recovery data.
