Live·Open questions in longevity research
All targets

Epigenetic mark

IRF4 half-site CpG methylation at the TGFB1 enhancer

A DNA methylation mark at the IRF4 half-site within the TGFB1 enhancer

Hypotheses on this target 1

IRF4 half-site CpG methylation at the TGFB1 enhancerSilencing. Hypotheses on this target 0Expression induction. Hypotheses on this target 0Reprogramming. Hypotheses on this target 0Direct measurement. Hypotheses on this target 11

Direct measurement1

  • Ageing installs the anti-fibrotic switch that spiny mice are born with

    Where this hypothesis actsCD163+ niche macrophages from aged (65–75) and young (20–30) human donors

    What is proposedMeasure methylation and its association with TGF-β1 secretion after SPM stimulation

    With whatInstrument or assay

    HowBisulfite sequencing of FACS-sorted CD163+ macrophages from bone marrow aspirates, paired with ex vivo SPM stimulation and TGF-β1 ELISA

    Possible resultExpected >60% methylation in aged donors and <10% in young donors, inversely correlated with TGF-β1 secretion

    2026-09-14