Synchronized renewal sustains barrier-loss cycles

The hypothesis asks if synchronized skin cells weaken the skin's water-loss barrier at constant drug concentration. Microbe-free organotypic skin, lab-grown skin tissue, would get one exposure to align cell maturation. At the surface, a cohort, a group of cells maturing together, would form outer layer and shed together. The hypothesis predicts at least three undiminished barrier-loss waves. Shedding peaks should precede water-loss peaks, timed by cohort transit. Spreading the first exposure should reduce wave amplitude at the same dose and similar cell divisions. Persistent waves would support this mechanism; waves fading after one cohort would reject its strong form. Testing needs lineage labels, division imaging, serial barrier tests and spatial drug measurements.